The Complete Amino Acid Sequence of ( Pltytolacca americana ) Chitinase - Bfrom the Leayes of Pokeweed
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چکیده
The complete amino acid sequence of pokeweed leaf chitinase-B (PLC-B) has been determined by first sequencing all 19 try, ptic peptides deriyed from the redu ¢ ed and S-carboxy, methylated (RCm-) PLC-B and then connecting them by analyzing the chymotryptic peptides from three fragments produced by cyanogen bromide cleavage of RCm-PLC-B. PLC-B consists of 274 amino acid residues and has a molecular mass of 29,473Da. Six cysteine residues are linked by disulfide bonds between Cys20 and Cys67, Cys50 and Cys57, and Cys159 and Cys188. From 58-68'ua sequence homology of PI.C-B with fiye class III chitinases, it was concluded that PLC-B is a basic class III chitinase.
منابع مشابه
The Complete Amino Acid Sequence of ( Pltytolacca americana ) Chitinase - Bfrom
The complete amino acid sequence of pokeweed leaf chitinase-B (PLC-B) has been determined by first sequencing all 19 try, ptic peptides deriyed from the redu ¢ ed and S-carboxy, methylated (RCm-) PLC-B and then connecting them by analyzing the chymotryptic peptides from three fragments produced by cyanogen bromide cleavage of RCm-PLC-B. PLC-B consists of 274 amino acid residues and has a molecu...
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